I'll just give you my take, since I couldn't really find anything concrete.
二刀流 is "two-sword style" (because of 流), so it's more like the technique itself. The most straightforward.
両刀使い is a "user" of two swords (literally). I think it's more casual. More emphasis on the person.
Pretty sure 両刀 is just the shorthand for 両刀使い
I think the latter 2 can lean more into the "bisexual person" meaning (especially 両刀). To my knowledge 二刀流 isn't really used like that (despite the dictionary entry). Pretty sure it's basically the "correct" pick for "dual-wielding" (usually swords) in most cases.
Hopefully someone else can correct me, or provide additional information :)
As soon as I saw 二刀流(にとうりゅう) , I was like "Wait, にとうりゅう, I heard that somewhere" And sure enough, like ギョルギ九十三 said, it's used to talk about the different styles/schools of using two sword including... 宮本武蔵's (Musashi Miyamoto) famous 二天一流(にてんいちりゅう) (probably heard it from that).
As for 両刀使い(りょうとうづかい), the first meaning is just 大小の刀のこと or 太刀と脇差のこと (tachi and wakizashi). So it's ambiguously about two sword (in japanese context -> tachi and wakizashi). Literaly or figuratively, whether it's about the objects, the styles or the user (as well as all the other definitions). So it seems to be more general than 二刀流 (which don't seems to be used to talk about the user).
And for 両刀(りょうとう), a few dictionaries just say "大小の刀。刀と脇差。" in contrast. Others copy-paste the definition of 二刀流 or 両刀遣い or say that it's a abbreviation of one of the two. So, you probably will have to ask or guess from the context. At the same, people tend to "contrast" words in those case or the words tend to get contrastive meaning/usage with time. Like sheep and mutton in english. But that's just a guess based on the fact that there are a few dictionaries that defined it with "大小の刀。刀と脇差。". Just ask or guess if you're unsure.
At least, that what I found from my research :3 Now, I'm gonna add those words to my schedule (I even had a conversation about 宮本武蔵 a few day ago, god dammit, that would have been perfect to practice those words )
